Acquired resistance occurs when a particular microorganism obtains the ability to resist the activity of an antimicrobial agent to which it was previously susceptible. This can result from the mutation of genes involved in normal physiological processes and cellular structures, from the acquisition of foreign resistance genes or from a combination of these two mechanisms.
Unlike intrinsic resistance, traits associated with acquired resistance are found only in some strains or subpopulations of each particular bacterial species. Acquired resistance results from successful gene change and/or exchange that may involve mutation or horizontal gene transfer via transformation, transduction or conjugation.
A mutation is a spontaneous change in the DNA sequence within the gene that may lead to a change in the trait which it codes for. Any change in a single base pair may lead to a corresponding change in one or more of the amino acids for which it codes, which can then change the enzyme or cell structure that consequently changes the affinity or effective activity of the targeted antimicrobials. In prokaryotic genomes, mutations frequently occur due to base changes caused by exogenous agents, DNA polymerase errors, deletions, insertions and duplications. For prokaryotes, there is a constant rate of spontaneous mutation of about 0.0033 mutations per DNA replication that is relatively uniform for a diverse spectrum of organisms. The mutation rate for individual genes varies significantly among and within gene
Many of the antibiotic resistance genes are carried on plasmids, transposons or integrons that can act as vectors that transfer these genes to other members of the same bacterial species, as well l as to bacteria in another genus or species.
These genetic mechanisms give to the bacteria some functional capacities that permit them to survive, the main ones are:
Link to European IC/HH Core Competencies: Area 4. Infection control activities